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Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

Overview of attention for article published in Journal of Experimental Botany, May 2016
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  • Above-average Attention Score compared to outputs of the same age (56th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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Citations

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39 Mendeley
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Title
Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
Published in
Journal of Experimental Botany, May 2016
DOI 10.1093/jxb/erw185
Pubmed ID
Authors

Carla Coluccio Leskow, Laura Kamenetzky, Pia Guadalupe Dominguez, José Antonio Díaz Zirpolo, Toshihiro Obata, Hernán Costa, Marcelo Martí, Oscar Taboga, Joost Keurentjes, Ronan Sulpice, Hirofumi Ishihara, Mark Stitt, Alisdair Robert Fernie, Fernando Carrari

Abstract

Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 23%
Researcher 8 21%
Student > Bachelor 7 18%
Student > Doctoral Student 4 10%
Student > Master 3 8%
Other 2 5%
Unknown 6 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 62%
Biochemistry, Genetics and Molecular Biology 7 18%
Unspecified 1 3%
Unknown 7 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2017.
All research outputs
#6,496,611
of 12,028,010 outputs
Outputs from Journal of Experimental Botany
#2,276
of 4,034 outputs
Outputs of similar age
#115,533
of 278,877 outputs
Outputs of similar age from Journal of Experimental Botany
#54
of 117 outputs
Altmetric has tracked 12,028,010 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,034 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,877 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 117 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.