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Rab5 is necessary for the biogenesis of the endolysosomal system in vivo

Overview of attention for article published in Nature, May 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

blogs
1 blog
twitter
3 tweeters
f1000
1 research highlight platform

Citations

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198 Dimensions

Readers on

mendeley
353 Mendeley
Title
Rab5 is necessary for the biogenesis of the endolysosomal system in vivo
Published in
Nature, May 2012
DOI 10.1038/nature11133
Pubmed ID
Authors

Anja Zeigerer, Jerome Gilleron, Roman L. Bogorad, Giovanni Marsico, Hidenori Nonaka, Sarah Seifert, Hila Epstein-Barash, Satya Kuchimanchi, Chang Geng Peng, Vera M. Ruda, Perla Del Conte-Zerial, Jan G. Hengstler, Yannis Kalaidzidis, Victor Koteliansky, Marino Zerial

Abstract

An outstanding question is how cells control the number and size of membrane organelles. The small GTPase Rab5 has been proposed to be a master regulator of endosome biogenesis. Here, to test this hypothesis, we developed a mathematical model of endosome dependency on Rab5 and validated it by titrating down all three Rab5 isoforms in adult mouse liver using state-of-the-art RNA interference technology. Unexpectedly, the endocytic system was resilient to depletion of Rab5 and collapsed only when Rab5 decreased to a critical level. Loss of Rab5 below this threshold caused a marked reduction in the number of early endosomes, late endosomes and lysosomes, associated with a block of low-density lipoprotein endocytosis. Loss of endosomes caused failure to deliver apical proteins to the bile canaliculi, suggesting a requirement for polarized cargo sorting. Our results demonstrate for the first time, to our knowledge, the role of Rab5 as an endosome organizer in vivo and reveal the resilience mechanisms of the endocytic system.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 353 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 9 3%
United States 5 1%
United Kingdom 3 <1%
Australia 1 <1%
Norway 1 <1%
Italy 1 <1%
France 1 <1%
India 1 <1%
Netherlands 1 <1%
Other 5 1%
Unknown 325 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 98 28%
Researcher 88 25%
Student > Master 42 12%
Student > Bachelor 37 10%
Student > Doctoral Student 17 5%
Other 52 15%
Unknown 19 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 176 50%
Biochemistry, Genetics and Molecular Biology 64 18%
Medicine and Dentistry 28 8%
Neuroscience 16 5%
Chemistry 9 3%
Other 34 10%
Unknown 26 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2013.
All research outputs
#761,906
of 7,356,675 outputs
Outputs from Nature
#22,721
of 45,250 outputs
Outputs of similar age
#9,298
of 91,288 outputs
Outputs of similar age from Nature
#481
of 762 outputs
Altmetric has tracked 7,356,675 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 45,250 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 69.2. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 91,288 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 762 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.