Title |
Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes
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Published in |
Genome Biology, March 2012
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DOI | 10.1186/gb-2012-13-3-r23 |
Pubmed ID | |
Authors |
Georgia Giannoukos, Dawn M Ciulla, Katherine Huang, Brian J Haas, Jacques Izard, Joshua Z Levin, Jonathan Livny, Ashlee M Earl, Dirk Gevers, Doyle V Ward, Chad Nusbaum, Bruce W Birren, Andreas Gnirke |
Abstract |
We have developed a process for transcriptome analysis of bacterial communities that accommodates both intact and fragmented starting RNA and combines efficient rRNA removal with strand-specific RNA-seq. We applied this approach to an RNA mixture derived from three diverse cultured bacterial species and to RNA isolated from clinical stool samples. The resulting expression profiles were highly reproducible, enriched up to 40-fold for non-rRNA transcripts, and correlated well with profiles representing undepleted total RNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 33% |
Unknown | 4 | 67% |
Demographic breakdown
Type | Count | As % |
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Scientists | 4 | 67% |
Members of the public | 2 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 30 | 4% |
Germany | 4 | <1% |
France | 4 | <1% |
Belgium | 4 | <1% |
United Kingdom | 4 | <1% |
Spain | 3 | <1% |
Japan | 2 | <1% |
Estonia | 2 | <1% |
Mexico | 2 | <1% |
Other | 19 | 2% |
Unknown | 732 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 232 | 29% |
Student > Ph. D. Student | 195 | 24% |
Student > Master | 73 | 9% |
Student > Bachelor | 55 | 7% |
Professor > Associate Professor | 44 | 5% |
Other | 137 | 17% |
Unknown | 70 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 443 | 55% |
Biochemistry, Genetics and Molecular Biology | 126 | 16% |
Immunology and Microbiology | 36 | 4% |
Environmental Science | 29 | 4% |
Medicine and Dentistry | 19 | 2% |
Other | 56 | 7% |
Unknown | 97 | 12% |