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| Title |
Bioactive Constituents of Zanthoxylum rhetsa Bark and Its Cytotoxic Potential against B16-F10 Melanoma Cancer and Normal Human Dermal Fibroblast (HDF) Cell Lines
|
|---|---|
| Published in |
Molecules, May 2016
|
| DOI | 10.3390/molecules21060652 |
| Pubmed ID | |
| Authors |
Ramesh Santhanam, Syahida Ahmad, Faridah Abas, Intan Safinar Ismail, Yaya Rukayadi, Muhammad Tayyab Akhtar, Khozirah Shaari |
| Abstract |
Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 62 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 16% |
| Lecturer | 6 | 10% |
| Student > Ph. D. Student | 5 | 8% |
| Student > Master | 4 | 6% |
| Professor | 3 | 5% |
| Other | 15 | 24% |
| Unknown | 19 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 16% |
| Chemistry | 9 | 15% |
| Biochemistry, Genetics and Molecular Biology | 7 | 11% |
| Medicine and Dentistry | 6 | 10% |
| Unspecified | 3 | 5% |
| Other | 4 | 6% |
| Unknown | 23 | 37% |