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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Scaling-Up of Dental Pulp Stem Cells Isolated from Multiple Niches
|
|---|---|
| Published in |
PLOS ONE, June 2012
|
| DOI | 10.1371/journal.pone.0039885 |
| Pubmed ID | |
| Authors |
Nelson F. Lizier, Alexandre Kerkis, Cícera M. Gomes, Josimeri Hebling, Camila F. Oliveira, Arnold I. Caplan, Irina Kerkis |
| Abstract |
Dental pulp (DP) can be extracted from child's primary teeth (deciduous), whose loss occurs spontaneously by about 5 to 12 years. Thus, DP presents an easy accessible source of stem cells without ethical concerns. Substantial quantities of stem cells of an excellent quality and at early (2-5) passages are necessary for clinical use, which currently is a problem for use of adult stem cells. Herein, DPs were cultured generating stem cells at least during six months through multiple mechanical transfers into a new culture dish every 3-4 days. We compared stem cells isolated from the same DP before (early population, EP) and six months after several mechanical transfers (late population, LP). No changes, in both EP and LP, were observed in morphology, expression of stem cells markers (nestin, vimentin, fibronectin, SH2, SH3 and Oct3/4), chondrogenic and myogenic differentiation potential, even after cryopreservation. Six hours after DP extraction and in vitro plating, rare 5-bromo-2'-deoxyuridine (BrdU) positive cells were observed in pulp central part. After 72 hours, BrdU positive cells increased in number and were found in DP periphery, thus originating a multicellular population of stem cells of high purity. Multiple stem cell niches were identified in different zones of DP, because abundant expression of nestin, vimentin and Oct3/4 proteins was observed, while STRO-1 protein localization was restricted to perivascular niche. Our finding is of importance for the future of stem cell therapies, providing scaling-up of stem cells at early passages with minimum risk of losing their "stemness". |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Brazil | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Colombia | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 106 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 21 | 19% |
| Student > Master | 17 | 16% |
| Student > Ph. D. Student | 15 | 14% |
| Researcher | 11 | 10% |
| Student > Doctoral Student | 7 | 6% |
| Other | 19 | 18% |
| Unknown | 18 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 32 | 30% |
| Agricultural and Biological Sciences | 21 | 19% |
| Biochemistry, Genetics and Molecular Biology | 16 | 15% |
| Materials Science | 3 | 3% |
| Engineering | 3 | 3% |
| Other | 9 | 8% |
| Unknown | 24 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2022.
All research outputs
#3,221,752
of 25,867,969 outputs
Outputs from PLOS ONE
#39,829
of 225,568 outputs
Outputs of similar age
#20,211
of 178,222 outputs
Outputs of similar age from PLOS ONE
#633
of 3,995 outputs
Altmetric has tracked 25,867,969 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 225,568 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.9. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 178,222 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 3,995 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.