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In Situ Hybridization Protocols

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Cover of 'In Situ Hybridization Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Guidelines for the Optimization and Validation of In Situ Hybridization
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    Chapter 2 Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization
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    Chapter 3 Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories
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    Chapter 4 Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probes
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    Chapter 5 Visualizing Genome Reorganization Using 3D DNA FISH
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    Chapter 6 MicroRNA In Situ Hybridization in Paraffin-Embedded Cultured Cells
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    Chapter 7 Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope™ in Cultured Cells
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    Chapter 8 Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0)
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    Chapter 9 Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0)
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    Chapter 10 Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Within Whole-Mount Vertebrate Embryos: qHCR and dHCR Imaging (v3.0)
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    Chapter 11 Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues
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    Chapter 12 RNAscope™ Multiplex Detection in Zebrafish
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    Chapter 13 Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope ®
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    Chapter 14 Automated ISH for Validated Histological Mapping of Lowly Expressed Genes
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    Chapter 15 Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping
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    Chapter 16 Automated Co-in Situ Hybridization and Immunofluorescence Using Archival Tumor Tissue
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    Chapter 17 Automated Five-Color Multiplex Co-detection of MicroRNA and Protein Expression in Fixed Tissue Specimens
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    Chapter 18 Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets
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    Chapter 19 Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscope™ In Situ Hybridization and Immunofluorescence Protocol
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    Chapter 20 In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections
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    Chapter 21 GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue
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    Chapter 22 In Situ Point Mutation Detection in FFPE Colorectal Cancers Using the BaseScope Assay
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    Chapter 23 Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells
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    Chapter 24 Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization
Attention for Chapter 20: In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections
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Chapter title
In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections
Chapter number 20
Book title
In Situ Hybridization Protocols
Published in
Methods in molecular biology, May 2020
DOI 10.1007/978-1-0716-0623-0_20
Pubmed ID
Book ISBNs
978-1-07-160622-3, 978-1-07-160623-0
Authors

Hilscher, Markus M., Gyllborg, Daniel, Yokota, Chika, Nilsson, Mats, Markus M. Hilscher, Daniel Gyllborg, Chika Yokota, Mats Nilsson

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 3 14%
Student > Master 3 14%
Student > Bachelor 3 14%
Student > Doctoral Student 2 10%
Student > Ph. D. Student 2 10%
Other 2 10%
Unknown 6 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 29%
Unspecified 3 14%
Agricultural and Biological Sciences 2 10%
Neuroscience 2 10%
Computer Science 1 5%
Other 1 5%
Unknown 6 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2020.
All research outputs
#13,678,871
of 23,207,489 outputs
Outputs from Methods in molecular biology
#3,698
of 13,305 outputs
Outputs of similar age
#195,065
of 386,673 outputs
Outputs of similar age from Methods in molecular biology
#16
of 76 outputs
Altmetric has tracked 23,207,489 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,305 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,673 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.