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Genotyping of Chimerical BCR-ABL1 RNA in Chronic Myeloid Leukemia by Integrated DNA Chip

Overview of attention for article published in The Journal of Molecular Diagnostics, September 2012
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Title
Genotyping of Chimerical BCR-ABL1 RNA in Chronic Myeloid Leukemia by Integrated DNA Chip
Published in
The Journal of Molecular Diagnostics, September 2012
DOI 10.1016/j.jmoldx.2012.04.003
Pubmed ID
Authors

Jong-Hun Kang, Hyun-Gyung Goh, Sang-Ho Chae, Sung-Yong Kim, Dong-Wook Kim, Chi-Bom Chae

Abstract

Chronic myelogenous leukemia (CML) and Philadelphia chromosome-positive (Ph(+)) acute lymphoblastic leukemia (ALL) are associated with fusion of the BCR and ABL1 genes by chromosome translocation. The chimerical BCR-ABL1 gene encodes different fusion proteins that vary in size, depending on the breakpoint in the BCR region. Different types of fusion genes in CML and Ph(+) ALL are thought to be related to the clinical course and outcome of each patient. Currently, the genotypes are determined by PCR, followed by gel electrophoresis or DNA sequencing (among other methodologies). Our major aim was to develop a diagnostic method for CML genotyping by means of an integrated process of DNA microarray. Here, we describe a method of integrated multiplex reverse transcription, asymmetric PCR, and hybridization, all in the same reaction mixture in a chamber assembled on the surface of capture oligonucleotide probes immobilized on a glass slide. The integrated system successfully identified the four predominant types of chimerical BCR-ABL1 RNA (b3a2, b2a2, e1a2, and c3a2), which together account for 98% of CML cases. The integrated multiplex system also had a high sensitivity of detection (as little as 200 molecules of target RNA molecules). The integrated process saves time and effort, and it also the advantage of minimizing the steps needed for automated detection of different types of chimerical CML RNA.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer 1 25%
Student > Ph. D. Student 1 25%
Researcher 1 25%
Unspecified 1 25%
Readers by discipline Count As %
Unspecified 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%
Nursing and Health Professions 1 25%
Agricultural and Biological Sciences 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2012.
All research outputs
#10,853,962
of 12,246,070 outputs
Outputs from The Journal of Molecular Diagnostics
#724
of 752 outputs
Outputs of similar age
#99,553
of 115,898 outputs
Outputs of similar age from The Journal of Molecular Diagnostics
#6
of 7 outputs
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