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Endocannabinoid Signaling

Overview of attention for book
Cover of 'Endocannabinoid Signaling'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
  3. Altmetric Badge
    Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
  4. Altmetric Badge
    Chapter 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS
  5. Altmetric Badge
    Chapter 4 Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
  6. Altmetric Badge
    Chapter 5 Assay of CB1 Receptor Binding
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    Chapter 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
  8. Altmetric Badge
    Chapter 7 Endocannabinoid Signaling
  9. Altmetric Badge
    Chapter 8 A Functional Assay for GPR55: Envision Protocol
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    Chapter 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 10 Assay of GTPγS Binding in Autoradiography
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    Chapter 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
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    Chapter 12 Assay of NAT Activity
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    Chapter 13 Assay of NAPE-PLD Activity
  15. Altmetric Badge
    Chapter 14 Assay of FAAH Activity
  16. Altmetric Badge
    Chapter 15 Assay of NAAA Activity
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    Chapter 16 Assay of DAGLα/β Activity
  18. Altmetric Badge
    Chapter 17 Endocannabinoid Signaling
  19. Altmetric Badge
    Chapter 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
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    Chapter 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
  21. Altmetric Badge
    Chapter 20 Assay of Endocannabinoid Uptake
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    Chapter 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
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    Chapter 22 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 23 Assay of Endocannabinoid Oxidation by Cytochrome P450
  25. Altmetric Badge
    Chapter 24 Assessing Gene Expression of the Endocannabinoid System
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    Chapter 25 Western Blotting of the Endocannabinoid System
  27. Altmetric Badge
    Chapter 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
  28. Altmetric Badge
    Chapter 27 Measuring ECS Interaction with Biomembranes
  29. Altmetric Badge
    Chapter 28 Visualization of Endocannabinoids in the Cell
Attention for Chapter 22: Oxygenation of Anandamide by Lipoxygenases
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Chapter title
Oxygenation of Anandamide by Lipoxygenases
Chapter number 22
Book title
Endocannabinoid Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3539-0_22
Pubmed ID
Book ISBNs
978-1-4939-3537-6, 978-1-4939-3539-0
Authors

Guus van Zadelhoff, Mario van der Stelt

Editors

Mauro Maccarrone

Abstract

The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase, monoacylglycerol lipase, and α,β-hydrolases 6 and 12, but they also serve as substrates for cyclooxygenases and lipoxygenases. These enzymes oxygenate the 1Z,4Z-pentadiene system of the arachidonic acid backbone of endocannabinoids, thereby giving rise to an entirely new array of bioactive lipids. Hereby, a protocol is provided for the enzymatic synthesis, purification, and characterization of various oxygenated metabolites of anandamide generated by lipoxygenases, which enables the biological study and detection of these metabolites.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 33%
Other 1 33%
Unknown 1 33%
Readers by discipline Count As %
Chemistry 1 33%
Medicine and Dentistry 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2017.
All research outputs
#15,376,252
of 22,875,477 outputs
Outputs from Methods in molecular biology
#5,350
of 13,131 outputs
Outputs of similar age
#230,972
of 393,698 outputs
Outputs of similar age from Methods in molecular biology
#544
of 1,471 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,698 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.