Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Chapter 1 The High-Throughput Sequencing Technologies Triple-W Discussion: Why Use HTS, What Is the Optimal HTS Method to Use, and Which Data Analysis Workflow to Follow

Chapter 2 Whole-Genome Sequencing Recommendations

Chapter 3 Targeted DNA Region Re-sequencing

Chapter 4 Transcriptome Profiling Strategies

Chapter 5 Differential mRNA Alternative Splicing

Chapter 7 Analysis of Long Noncoding RNAs in RNA-Seq Data

Chapter 8 Ribosome Profiling

Chapter 9 Genome-Wide Analysis of DNA Methylation Patterns by High-Throughput Sequencing

Chapter 10 Characterization of DNA-Protein Interactions: Design and Analysis of ChIP-Seq Experiments

Chapter 11 PAR-CLIP: A Genomic Technique to Dissect RNA-Protein Interactions

Chapter 12 Metagenomic Design and Sequencing

Chapter 13 A Hitchhiker’s Guide to Metatranscriptomics

Chapter 14 Eukaryotic Single-Cell mRNA Sequencing

Chapter 15 Eukaryotic Single-Cell DNA Sequencing

Chapter 16 Submitting Data to a Public Repository, the Final Step of a Successful HTS Experiment