| Chapter title |
Single-molecule optical-trapping measurements with DNA anchored to an array of gold nanoposts.
|
|---|---|
| Chapter number | 18 |
| Book title |
Spectroscopic Methods of Analysis
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-806-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-805-4, 978-1-61779-806-1
|
| Authors |
Paik DH, Perkins TT, D. Hern Paik, Thomas T. Perkins, Paik, D. Hern, Perkins, Thomas T. |
| Abstract |
Gold-thiol chemistry is one of the most successful chemistries for conjugating biomolecules to surfaces, but such chemistry has not been exploited in optical-trapping experiments because of laser-induced ablation of gold. In this work, we describe a method to combine these two separate technologies without undue heating using DNA anchored to gold nanostructures (r = 50-250 nm; h ≈ 20 nm). Moreover, we demonstrate a quantitative and mechanically robust (>100 pN) optical-trapping assay. By using three dithiol phosphoramidites (DTPAs) incorporated into a polymerase chain reaction (PCR) primer, the gold-DNA bond remained stable in the presence of excess thiolated compounds. This chemical robustness allowed us to reduce nonspecific sticking by passivating the unreacted gold with methoxy-(polyethylene glycol)-thiol (mPEG-SH). Overall, this surface conjugation of biomolecules onto an ordered array of gold nanostructures by chemically and mechanically robust bonds provides a unique way to carry out spatially controlled, repeatable measurements of single molecules. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |