Phytoplasma

Table of Contents

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   Book Overview
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   Chapter 1 The phytoplasmas: an introduction.
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   Chapter 2 Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus.
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   Chapter 3 Micropropagation and maintenance of phytoplasmas in tissue culture.
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   Chapter 4 Dodder transmission of phytoplasmas.
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   Chapter 5 Insect maintenance and transmission.
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   Chapter 6 Capturing insect vectors of phytoplasmas.
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   Chapter 7 Insect vector transmission assays.
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   Chapter 8 Molecular identification of phytoplasma vector species.
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    Chapter 9 Dienes' staining and light microscopy for phytoplasma visualization.
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    Chapter 10 DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas.
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    Chapter 11 Visualization of phytoplasmas using electron microscopy.
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    Chapter 12 Automated DNA extraction for large numbers of plant samples.
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    Chapter 13 DNA extraction from arborescent monocots and how to deal with other challenging hosts.
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    Chapter 14 Nested PCR and RFLP Analysis Based on the 16S rRNA Gene.
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    Chapter 15 PCR and RFLP Analyses Based on the Ribosomal Protein Operon.
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    Chapter 16 Tuf and secY PCR Amplification and Genotyping of Phytoplasmas.
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    Chapter 17 PCR Analysis of Phytoplasmas Based on the secA Gene.
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    Chapter 18 Single-strand conformation polymorphism analysis for differentiating phytoplasma strains.
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    Chapter 19 Microarrays for Universal Detection and Identification of Phytoplasmas
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    Chapter 20 T-RFLP for Detection and Identification of Phytoplasmas in Plants.
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    Chapter 21 Real-time PCR for universal phytoplasma detection and quantification.
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    Chapter 22 A real-time PCR detection system for the bois noir and flavescence dorée phytoplasmas and quantification of the target DNA.
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    Chapter 23 Real-time PCR for specific detection of three phytoplasmas from the apple proliferation group.
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    Chapter 24 Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions.
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    Chapter 25 In-field diagnostics using loop-mediated isothermal amplification.
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    Chapter 26 DNA bar-coding for phytoplasma identification.
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    Chapter 27 Phylogenetic analyses of phytoplasmas based on whole-genome comparison.
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    Chapter 28 The iPhyClassifier, an Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment.
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    Chapter 29 Phytoplasma proteomic analysis.
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    Chapter 30 Preparation of phytoplasma membrane recombinant proteins.
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    Chapter 31 Phytoplasma plasmid DNA extraction.
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    Chapter 32 Cesium chloride-bisbenzimide gradients for separation of phytoplasma and plant DNA.
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    Chapter 33 Pulsed-field gel electrophoresis for isolation of full-length phytoplasma chromosomes from plants.
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    Chapter 34 Mapping the phytoplasma chromosome.