Title |
A yeast expression system for functional and pharmacological studies of the malaria parasite Ca2+/H+ antiporter
|
---|---|
Published in |
Malaria Journal, August 2012
|
DOI | 10.1186/1475-2875-11-254 |
Pubmed ID | |
Authors |
J Enrique Salcedo-Sora, Steve A Ward, Giancarlo A Biagini |
Abstract |
Calcium (Ca²⁺) signalling is fundamental for host cell invasion, motility, in vivo synchronicity and sexual differentiation of the malaria parasite. Consequently, cytoplasmic free Ca²⁺ is tightly regulated through the co-ordinated action of primary and secondary Ca²⁺ transporters. Identifying selective inhibitors of Ca²⁺ transporters is key towards understanding their physiological role as well as having therapeutic potential, therefore screening systems to facilitate the search for potential inhibitors are a priority. Here, the methodology for the expression of a Calcium membrane transporter that can be scaled to high throughputs in yeast is presented. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 50% |
Nigeria | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Indonesia | 1 | 4% |
Slovenia | 1 | 4% |
Unknown | 24 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 27% |
Student > Master | 5 | 19% |
Student > Ph. D. Student | 4 | 15% |
Student > Postgraduate | 2 | 8% |
Student > Bachelor | 2 | 8% |
Other | 3 | 12% |
Unknown | 3 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 38% |
Biochemistry, Genetics and Molecular Biology | 5 | 19% |
Chemistry | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Economics, Econometrics and Finance | 1 | 4% |
Other | 3 | 12% |
Unknown | 4 | 15% |