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Plant MAP Kinases

Overview of attention for book
Cover of 'Plant MAP Kinases'

Table of Contents

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    Book Overview
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    Chapter 1 Transient expression in Arabidopsis leaf mesophyll protoplast system for cell-based functional analysis of MAPK cascades signaling.
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    Chapter 2 Quantification of stress-induced mitogen-activated protein kinase expressional dynamic using reverse transcription quantitative real-time PCR.
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    Chapter 3 Analysis of MAPK Activities Using MAPK-Specific Antibodies.
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    Chapter 4 Detection of protein phosphorylation and charge isoforms using vertical one-dimensional isoelectric focusing gels.
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    Chapter 5 Affinity-Based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™.
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    Chapter 6 Identification of Constitutively Active AtMPK6 Mutants Using a Functional Screen in Saccharomyces cerevisiae.
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    Chapter 7 Virus-induced gene silencing in plant MAPK research.
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    Chapter 8 RNA interference of plant MAPK cascades for functional studies.
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    Chapter 9 Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole-Mount Probes.
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    Chapter 10 Immunofluorescent Localization of MAPKs in Steedman's Wax Sections.
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    Chapter 11 Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies.
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    Chapter 12 Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases.
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    Chapter 13 Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1.
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    Chapter 14 In Vivo Phosphorylation of WRKY Transcription Factor by MAPK.
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    Chapter 15 Rapid mutagenesis-based analysis of phosphorylation sites in mitogen-activated protein kinase substrates.
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    Chapter 16 Yeast Two-Hybrid System for Dissecting the Rice MAPK Interactome.
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    Chapter 17 Experimental and analytical approaches to characterize plant kinases using protein microarrays.
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    Chapter 18 Protein Complexes Characterization in Arabidopsis thaliana by Tandem Affinity Purification Coupled to Mass Spectrometry Analysis.
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    Chapter 19 Quantitative Phosphoproteomic Analysis Using iTRAQ Method.
Attention for Chapter 16: Yeast Two-Hybrid System for Dissecting the Rice MAPK Interactome.
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Chapter title
Yeast Two-Hybrid System for Dissecting the Rice MAPK Interactome.
Chapter number 16
Book title
Plant MAP Kinases
Published in
Methods in molecular biology, May 2014
DOI 10.1007/978-1-4939-0922-3_16
Pubmed ID
24908130
Book ISBNs
978-1-4939-0921-6, 978-1-4939-0922-3
Authors

Raksha Singh, Sarmina Dangol, Nam-Soo Jwa

Editors

George Komis, Jozef Šamaj

Abstract

Protein-protein interactions are a preliminary but fundamental key to many biological systems. Identification of proteins that interact with particular bait not only contributes to a deeper understanding of bait protein function but also provides much information for the discovery of larger-scale interaction networks (interactome). Therefore, protein-protein interaction mapping is regarded as a widely accepted standardized functional genomics technique that provides comprehensive functional interpretation of previously uncharacterized proteins. A commonly used approach to detecting novel protein-protein interactions is the yeast two-hybrid system. In this chapter we describe in detail the protocols used to dissect the rice MAPK interactome, including the bait protein auto-activation test, identification of a rice MAPK interacting protein, confirmation of interaction by retransformation assay and characterization of the novel interacting protein.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 57%
Lecturer 1 14%
Student > Doctoral Student 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Agricultural and Biological Sciences 3 43%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2014.
All research outputs
#15,251,976
of 22,679,690 outputs
Outputs from Methods in molecular biology
#5,289
of 13,038 outputs
Outputs of similar age
#132,570
of 226,204 outputs
Outputs of similar age from Methods in molecular biology
#32
of 122 outputs
Altmetric has tracked 22,679,690 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,038 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 226,204 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 122 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.

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