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No involvement of alveolar macrophages in the initiation of carbon nanoparticle induced acute lung inflammation in mice

Overview of attention for article published in Particle and Fibre Toxicology, June 2016
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Title
No involvement of alveolar macrophages in the initiation of carbon nanoparticle induced acute lung inflammation in mice
Published in
Particle and Fibre Toxicology, June 2016
DOI 10.1186/s12989-016-0144-6
Pubmed ID
Authors

Shanze Chen, Renfu Yin, Kathrin Mutze, Youjia Yu, Shinji Takenaka, Melanie Königshoff, Tobias Stoeger

Abstract

Carbonaceous nanoparticles (CNP) represent a major constituent of urban particulate air pollution, and inhalation of high CNP levels has been described to trigger a pro-inflammatory response of the lung. While several studies identified specific particle characteristics driving respiratory toxicity of low-solubility and low-toxicity particles such as CNP, the major lung cell type, which initiates and drives that response, remains still uncertain. Since alveolar macrophages (AM) are known to effectively phagocytose inhaled particles and play a crucial role for the initiation of pulmonary inflammation caused by invading microbes, we aimed to determine their role for sterile stimuli such as CNP by profiling the primary alveolar cell compartments of the lung. We exposed C57BL/6 mice to 20 μg CNP by intratracheal instillation and comprehensively investigated the expression of the underlying mediators during a time span of 3 to 72 h in three different lung cell populations: CD45- (negative) structural cells, CD45+ (positive) leukocytes, and by BAL recovered cells. Bronchoalveolar lavage (BAL) analysis revealed an acute inflammatory response characterized by the most prominent culmination of neutrophil granulocytes from 12 to 24 h after instillation, which declined to basal levels by day 7. As early as 3 h after CNP exposure 50 % of the AM revealed particle laden. BAL concentrations and lung gene expression profiles of TNFα, and the neutrophil chemoattractants CXCL1,-2 and-5 preceded the neutrophil recruitment and showed highest levels after 12 h of CNP exposure, pointing to a significant activation of the inflammation-evoking lung cells at this point of time. AM, isolated from lungs 3 to 12 h after CNP instillation, however, did not show a pro-inflammatory signature. On the contrary, gene expression analysis of different lung cell populations isolated 12 h after CNP instillation revealed CD45-, mainly representing alveolar epithelial type II (ATII) cells as major producer of inflammatory CXCL cytokines. Particularly by CD45- cells expressed Cxcl5 proved to be the most abundant chemokine, being 12 h after CNP exposure 24 (±11) fold induced. Our data suggests that AM are noninvolved in the initiation of the inflammatory response. ATII cells, which induced highest CXCL levels early on, might in contrast be the driver of acute neutrophilic inflammation upon pulmonary CNP exposure.

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Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Unknown 40 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 24%
Student > Bachelor 6 15%
Researcher 6 15%
Student > Master 4 10%
Professor > Associate Professor 3 7%
Other 5 12%
Unknown 7 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 15%
Agricultural and Biological Sciences 5 12%
Environmental Science 3 7%
Medicine and Dentistry 3 7%
Immunology and Microbiology 2 5%
Other 11 27%
Unknown 11 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2020.
All research outputs
#12,524,536
of 15,766,637 outputs
Outputs from Particle and Fibre Toxicology
#340
of 466 outputs
Outputs of similar age
#185,064
of 264,929 outputs
Outputs of similar age from Particle and Fibre Toxicology
#1
of 1 outputs
Altmetric has tracked 15,766,637 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 466 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.1. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
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