Title |
Protection of Primary Dopaminergic Midbrain Neurons by GPR139 Agonists Supports Different Mechanisms of MPP+ and Rotenone Toxicity
|
---|---|
Published in |
Frontiers in Cellular Neuroscience, June 2016
|
DOI | 10.3389/fncel.2016.00164 |
Pubmed ID | |
Authors |
Kirsten Bayer Andersen, Jens Leander Johansen, Morten Hentzer, Garrick Paul Smith, Gunnar P. H. Dietz |
Abstract |
The G-protein coupled receptor 139 (GPR139) is expressed specifically in the brain in areas of relevance for motor control. GPR139 function and signal transduction pathways are elusive, and results in the literature are even contradictory. Here, we examined the potential neuroprotective effect of GPR139 agonism in primary culture models of dopaminergic (DA) neuronal degeneration. We find that in vitro GPR139 agonists protected primary mesencephalic DA neurons against 1-methyl-4-phenylpyridinium (MPP(+))-mediated degeneration. Protection was concentration-dependent and could be blocked by a GPR139 antagonist. However, the protection of DA neurons was not found against rotenone or 6-hydroxydopamine (6-OHDA) mediated degeneration. Our results support differential mechanisms of toxicity for those substances commonly used in Parkinson's disease (PD) models and potential for GPR139 agonists in neuroprotection. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 19% |
Student > Bachelor | 6 | 16% |
Student > Ph. D. Student | 5 | 14% |
Student > Doctoral Student | 4 | 11% |
Other | 3 | 8% |
Other | 3 | 8% |
Unknown | 9 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 16% |
Agricultural and Biological Sciences | 5 | 14% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Neuroscience | 4 | 11% |
Medicine and Dentistry | 3 | 8% |
Other | 4 | 11% |
Unknown | 11 | 30% |