| Chapter title |
In Vitro Methods for Studying the Mechanisms of Resistance to DNA-Damaging Therapeutic Drugs.
|
|---|---|
| Chapter number | 3 |
| Book title |
Cancer Drug Resistance
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3347-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3345-7, 978-1-4939-3347-1
|
| Authors |
Pasarat Khongkow, Anna K. Middleton, Jocelyn P.-M. Wong, Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes, Ana R. Gomes, Eric W.-F. Lam, Anna W. Middleton |
| Editors |
José Rueff, António Sebastião Rodrigues |
| Abstract |
Most commonly used anticancer drugs exert their effects mainly by causing DNA damage. The enhancement in DNA damage response (DDR) is considered a key mechanism that enables cancer cells to survive through eliminating the damaged DNA lesions and thereby developing resistance to DNA-damaging agents. This chapter describes the four experimental approaches for studying DDR and genotoxic drug resistance, including the use of γ-H2AX and comet assays to monitor DNA damage and repair capacity as well as the use of clonogenic and β-galactosidase staining assays to assess long-term cell fate after DNA-damaging treatment. Finally, we also present examples of these methods currently used in our laboratory for studying the role of FOXM1 in DNA damage-induced senescence and epirubicin resistance. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 22% |
| Student > Postgraduate | 1 | 11% |
| Lecturer | 1 | 11% |
| Unknown | 5 | 56% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 22% |
| Agricultural and Biological Sciences | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Unknown | 5 | 56% |