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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency
|
|---|---|
| Published in |
PLOS ONE, August 2012
|
| DOI | 10.1371/journal.pone.0043708 |
| Pubmed ID | |
| Authors |
Jayne M. Squirrell, Jimmy J. Fong, Carlos A. Ariza, Amber Mael, Kassondra Meyer, Nirupama K. Shevde, Avtar Roopra, Gary E. Lyons, Timothy J. Kamp, Kevin W. Eliceiri, Brenda M. Ogle |
| Abstract |
The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 9% |
| Portugal | 1 | 2% |
| Unknown | 39 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 27% |
| Researcher | 9 | 20% |
| Professor | 6 | 14% |
| Student > Master | 4 | 9% |
| Other | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 4 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 25% |
| Biochemistry, Genetics and Molecular Biology | 9 | 20% |
| Physics and Astronomy | 4 | 9% |
| Engineering | 4 | 9% |
| Medicine and Dentistry | 3 | 7% |
| Other | 7 | 16% |
| Unknown | 6 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2023.
All research outputs
#7,024,224
of 25,059,640 outputs
Outputs from PLOS ONE
#95,701
of 217,363 outputs
Outputs of similar age
#48,349
of 177,417 outputs
Outputs of similar age from PLOS ONE
#1,379
of 4,363 outputs
Altmetric has tracked 25,059,640 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 217,363 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.7. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 177,417 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 4,363 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.