@Mykalt45 @RTLeenay I don’t know if anything else. I remember the CRISPOR paper by Haussler found that the U6 vs other divide was the biggest driver out differences in guide effectiveness suggesting no one did anything else in 2016. https://t.co/DJ4ARtnp
@bioamara Por ej. No es igual usar plásmidos que produzcan los gRNAs que usarlos ya como una molécula sintetizada como RNP o junto al mRNA de Cas9. En este artículo detallan estas cosas muy bien: https://t.co/x8i7mc3Ihu
RT @crisprscan: @crisprflydesign @jjfroehlich @borauyar @N_Rajewsky I guess that different approaches can influence a lot in gRNA efficienc…
@crisprflydesign @jjfroehlich @borauyar @N_Rajewsky I guess that different approaches can influence a lot in gRNA efficiency predictions. Here it is shown that approaches rather than systems or models can be important when predicting gRNA activity. Not the