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Oxidative origin of sperm DNA fragmentation in the adult varicocele

Overview of attention for article published in International braz j urol, March 2021
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Title
Oxidative origin of sperm DNA fragmentation in the adult varicocele
Published in
International braz j urol, March 2021
DOI 10.1590/s1677-5538.ibju.2019.0827
Pubmed ID
Authors

Jessica Timóteo Jeremias, Larissa Berloffa Belardin, Fatima Kazue Okada, Mariana P. Antoniassi, Renato Fraietta, Ricardo Pimenta Bertolla, Paula Intasqui

Abstract

Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student's t test or a Mann-Whitney test. The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidative-induced DNA fragmentation. Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 25%
Researcher 2 17%
Student > Postgraduate 2 17%
Student > Master 2 17%
Unknown 3 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Agricultural and Biological Sciences 2 17%
Environmental Science 1 8%
Philosophy 1 8%
Unknown 3 25%