Report for: Expressed Sequence Tags (ESTs)

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Chapter title	Expressed Sequence Tags: An Overview
Chapter number	1
Book title	Expressed Sequence Tags (ESTs)
Published in	Methods in molecular biology, March 2009
DOI	10.1007/978-1-60327-136-3_1
Pubmed ID	19277571
Book ISBNs	978-1-58829-759-4, 978-1-60327-136-3
Authors	John Parkinson, Mark Blaxter, Parkinson J, Blaxter M, Parkinson, John, Blaxter, Mark
Abstract	Expressed sequence tags (ESTs) are fragments of mRNA sequences derived through single sequencing reactions performed on randomly selected clones from cDNA libraries. To date, over 45 million ESTs have been generated from over 1400 different species of eukaryotes. For the most part, EST projects are used to either complement existing genome projects or serve as low-cost alternatives for purposes of gene discovery. However, with improvements in accuracy and coverage, they are beginning to find application in fields such as phylogenetics, transcript profiling and proteomics. This volume provides practical details on the generation and analysis of ESTs. Chapters are presented which cover creation of cDNA libraries; generation and processing of sequence data; bioinformatics analysis of ESTs; and their application to phylogenetics and transcript profiling.

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Mendeley readers

The data shown below were compiled from readership statistics for 222 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Canada	2	<1%
United States	2	<1%
Brazil	2	<1%
Colombia	1	<1%
Germany	1	<1%
Mexico	1	<1%
Malaysia	1	<1%
Unknown	212	95%

Demographic breakdown

Readers by professional status	Count	As %
Student > Master	46	21%
Student > Ph. D. Student	41	18%
Student > Bachelor	40	18%
Researcher	20	9%
Student > Doctoral Student	8	4%
Other	20	9%
Unknown	47	21%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	86	39%
Biochemistry, Genetics and Molecular Biology	52	23%
Medicine and Dentistry	7	3%
Computer Science	5	2%
Chemistry	4	2%
Other	19	9%
Unknown	49	22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2014.

All research outputs

#20,235,415

of 22,761,738 outputs

Outputs from Methods in molecular biology

#9,864

of 13,088 outputs

Outputs of similar age

#90,632

of 94,237 outputs

Outputs of similar age from Methods in molecular biology

#32

of 36 outputs

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So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

Expressed Sequence Tags (ESTs)

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Mendeley readers

Geographical breakdown

Demographic breakdown

Attention Score in Context