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High-efficacy subcellular micropatterning of proteins using fibrinogen anchors

Overview of attention for article published in Journal of Cell Biology, January 2021
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

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1 blog
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2 Facebook pages

Citations

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18 Dimensions

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59 Mendeley
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Title
High-efficacy subcellular micropatterning of proteins using fibrinogen anchors
Published in
Journal of Cell Biology, January 2021
DOI 10.1083/jcb.202009063
Pubmed ID
Authors

Joseph L. Watson, Samya Aich, Benjamí Oller-Salvia, Andrew A. Drabek, Stephen C. Blacklow, Jason Chin, Emmanuel Derivery

Abstract

Protein micropatterning allows proteins to be precisely deposited onto a substrate of choice and is now routinely used in cell biology and in vitro reconstitution. However, drawbacks of current technology are that micropatterning efficiency can be variable between proteins and that proteins may lose activity on the micropatterns. Here, we describe a general method to enable micropatterning of virtually any protein at high specificity and homogeneity while maintaining its activity. Our method is based on an anchor that micropatterns well, fibrinogen, which we functionalized to bind to common purification tags. This enhances micropatterning on various substrates, facilitates multiplexed micropatterning, and dramatically improves the on-pattern activity of fragile proteins like molecular motors. Furthermore, it enhances the micropatterning of hard-to-micropattern cells. Last, this method enables subcellular micropatterning, whereby complex micropatterns simultaneously control cell shape and the distribution of transmembrane receptors within that cell. Altogether, these results open new avenues for cell biology.

X Demographics

X Demographics

The data shown below were collected from the profiles of 40 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 59 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 17%
Student > Master 9 15%
Student > Bachelor 6 10%
Researcher 5 8%
Other 3 5%
Other 8 14%
Unknown 18 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 37%
Agricultural and Biological Sciences 6 10%
Physics and Astronomy 2 3%
Chemistry 2 3%
Engineering 2 3%
Other 5 8%
Unknown 20 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 29. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2023.
All research outputs
#1,382,385
of 26,058,621 outputs
Outputs from Journal of Cell Biology
#575
of 12,081 outputs
Outputs of similar age
#37,781
of 532,701 outputs
Outputs of similar age from Journal of Cell Biology
#9
of 78 outputs
Altmetric has tracked 26,058,621 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,081 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.6. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 532,701 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 78 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.