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Sensitive detection of multiple pathogens using a single DNA probe

Overview of attention for article published in Biosensors & Bioelectronics, December 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

twitter
5 tweeters

Citations

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13 Dimensions

Readers on

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40 Mendeley
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Title
Sensitive detection of multiple pathogens using a single DNA probe
Published in
Biosensors & Bioelectronics, December 2016
DOI 10.1016/j.bios.2016.06.077
Pubmed ID
Authors

Noordiana Nordin, Nor Azah Yusof, Jaafar Abdullah, Son Radu, Roozbeh Hushiarian

Abstract

A simple but promising electrochemical DNA nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. The DNA probe was initially designed to have a complementary region in Vibrio parahaemolyticus (VP) genome and to make different hybridization patterns with other selected pathogens. The sensor was based on a screen printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs) and methylene blue (MB) was employed as the redox indicator binding better to single-stranded DNA. The immobilization and hybridization events were assessed using differential pulse voltammetry (DPV). The fabricated biosensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0×10(-9)-2.0×10(-13)M with a detection limit of 5.3×10(-12)M. The relative standard deviation for 6 replications of DPV measurement of 0.2µM complementary DNA was 4.88%. The fabricated DNA biosensor was considered stable and portable as indicated by a recovery of more than 80% after a storage period of 6 months at 4-45°C. Cross-reactivity studies against various food-borne pathogens showed a reliably sensitive detection of VP.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Iran, Islamic Republic of 1 3%
Australia 1 3%
Unknown 38 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 30%
Student > Master 7 18%
Researcher 5 13%
Student > Doctoral Student 4 10%
Lecturer 3 8%
Other 7 18%
Unknown 2 5%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 23%
Chemistry 9 23%
Agricultural and Biological Sciences 6 15%
Engineering 2 5%
Materials Science 2 5%
Other 8 20%
Unknown 4 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 July 2016.
All research outputs
#6,540,810
of 12,119,486 outputs
Outputs from Biosensors & Bioelectronics
#2,256
of 4,269 outputs
Outputs of similar age
#110,903
of 267,564 outputs
Outputs of similar age from Biosensors & Bioelectronics
#18
of 102 outputs
Altmetric has tracked 12,119,486 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,269 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,564 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 102 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.