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Live-cell super-resolution imaging with trimethoprim conjugates

Overview of attention for article published in Nature Methods, August 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

blogs
1 blog
patent
1 patent

Citations

dimensions_citation
308 Dimensions

Readers on

mendeley
452 Mendeley
citeulike
3 CiteULike
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Title
Live-cell super-resolution imaging with trimethoprim conjugates
Published in
Nature Methods, August 2010
DOI 10.1038/nmeth.1489
Pubmed ID
Authors

Richard Wombacher, Meike Heidbreder, Sebastian van de Linde, Michael P Sheetz, Mike Heilemann, Virginia W Cornish, Markus Sauer

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 452 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 11 2%
United States 10 2%
United Kingdom 8 2%
Japan 7 2%
France 4 <1%
Netherlands 3 <1%
Chile 1 <1%
India 1 <1%
Czechia 1 <1%
Other 9 2%
Unknown 397 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 133 29%
Student > Ph. D. Student 120 27%
Student > Master 34 8%
Professor > Associate Professor 28 6%
Student > Bachelor 26 6%
Other 64 14%
Unknown 47 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 175 39%
Chemistry 63 14%
Biochemistry, Genetics and Molecular Biology 54 12%
Physics and Astronomy 49 11%
Engineering 24 5%
Other 30 7%
Unknown 57 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2015.
All research outputs
#3,564,808
of 22,679,690 outputs
Outputs from Nature Methods
#2,736
of 4,889 outputs
Outputs of similar age
#15,006
of 94,404 outputs
Outputs of similar age from Nature Methods
#24
of 45 outputs
Altmetric has tracked 22,679,690 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,889 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 34.5. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,404 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.