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Ubiquitin Family Modifiers and the Proteasome

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Cover of 'Ubiquitin Family Modifiers and the Proteasome'

Table of Contents

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    Book Overview
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    Chapter 1 Three decades of studies to understand the functions of the ubiquitin family.
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    Chapter 2 Structure and evolution of ubiquitin and ubiquitin-related domains.
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    Chapter 3 Deciphering tissue-specific ubiquitylation by mass spectrometry.
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    Chapter 4 Analysis of Cellular SUMO and SUMO-Ubiquitin Hybrid Conjugates.
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    Chapter 5 Recombinant reconstitution of sumoylation reactions in vitro.
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    Chapter 6 Reconstitution of SUMO-Dependent Ubiquitylation In Vitro.
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    Chapter 7 Detection and Analysis of FAT10 Modification.
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    Chapter 8 Isolation of NEDDylated Proteins in Human Cells.
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    Chapter 9 The ISG15 Conjugation System.
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    Chapter 10 Pupylation: proteasomal targeting by a protein modifier in bacteria.
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    Chapter 11 Role of UbL Family Modifiers and Their Binding Proteins in Cell Signaling.
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    Chapter 12 Ubiquitin Family Modifiers and the Proteasome
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    Chapter 13 Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation.
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    Chapter 14 Measuring rates of ubiquitin chain formation as a functional readout of ligase activity.
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    Chapter 15 Synthesis and analysis of k11-linked ubiquitin chains.
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    Chapter 16 Synthesis and analysis of linear ubiquitin chains.
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    Chapter 17 Detection and Quantitation of SUMO Chains by Mass Spectrometry
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    Chapter 18 Bioinformatical Detection of Recognition Factors for Ubiquitin and SUMO.
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    Chapter 19 Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners.
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    Chapter 20 Identifying and studying ubiquitin receptors by NMR.
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    Chapter 21 Exploring the Role of p97 and Its UBX-Domain Cofactors Through Identification of Their Interacting Proteins.
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    Chapter 22 Assembly and function of the proteasome.
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    Chapter 23 Using Native Gel Electrophoresis and Phosphofluoroimaging to Analyze GFP-Tagged Proteasomes.
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    Chapter 24 Disulfide engineering to map subunit interactions in the proteasome and other macromolecular complexes.
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    Chapter 25 Using DNA damage sensitivity phenotypes to characterize mutations affecting proteasome function.
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    Chapter 26 Analysing properties of proteasome inhibitors using kinetic and x-ray crystallographic studies.
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    Chapter 27 Immunoproteasome-specific inhibitors and their application.
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    Chapter 28 Binding of ubiquitin conjugates to proteasomes as visualized with native gels.
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    Chapter 29 Affinity Purification of Mammalian 26S Proteasomes Using an Ubiquitin-Like Domain.
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    Chapter 30 Using siRNA Techniques to Dissect Proteasome Assembly Pathways in Mammalian Cells.
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    Chapter 31 Reconstitution of PA700, the 19S Regulatory Particle, from Purified Precursor Complexes.
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    Chapter 32 Cellular responses to misfolded proteins and protein aggregates.
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    Chapter 33 Live-cell imaging of ubiquitin-proteasome system function.
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    Chapter 34 Analysis of chaperone-assisted ubiquitylation.
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    Chapter 35 Use of CPY* and Its Derivatives to Study Protein Quality Control in Various Cell Compartments.
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    Chapter 36 Assays to Measure ER-Associated Degradation in Yeast.
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    Chapter 37 SDS-PAGE Techniques to Study Ubiquitin-Like Conjugation Systems in Yeast Autophagy.
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    Chapter 38 Analysis of Ubiquitin-Dependent Proteolysis in Caenorhabditis elegans.
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    Chapter 39 Structural insights into functional modes of proteins involved in ubiquitin family pathways.
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    Chapter 40 Ubiquitin Family Modifiers and the Proteasome
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    Chapter 41 Ubiquitin Family Modifiers and the Proteasome
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    Chapter 42 Synthesis of atypical diubiquitin chains.
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    Chapter 43 TIPI: TEV Protease-Mediated Induction of Protein Instability.
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    Chapter 44 PROTAC-Induced Proteolytic Targeting
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    Chapter 45 Formation of nondegradable forked ubiquitin conjugates by ring-finger ligases and its prevention by s5a.
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    Chapter 46 S5a/Rpn10, a UIM-Protein, as a Universal Substrate for Ubiquitination.
Attention for Chapter 20: Identifying and studying ubiquitin receptors by NMR.
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Chapter title
Identifying and studying ubiquitin receptors by NMR.
Chapter number 20
Book title
Ubiquitin Family Modifiers and the Proteasome
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-474-2_20
Pubmed ID
Book ISBNs
978-1-61779-473-5, 978-1-61779-474-2
Authors

Chen X, Walters KJ, Xiang Chen, Kylie J. Walters

Editors

R. Jürgen Dohmen, Martin Scheffner

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 10%
Ukraine 1 10%
Unknown 8 80%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Researcher 3 30%
Professor 1 10%
Student > Postgraduate 1 10%
Unknown 1 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 40%
Agricultural and Biological Sciences 4 40%
Medicine and Dentistry 1 10%
Unknown 1 10%