Title |
Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells
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Published in |
BMC Biotechnology, October 2012
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DOI | 10.1186/1472-6750-12-72 |
Pubmed ID | |
Authors |
Yasuhiko Sumitomo, Hiroaki Higashitsuji, Hisako Higashitsuji, Yu Liu, Takanori Fujita, Toshiharu Sakurai, Marco M Candeias, Katsuhiko Itoh, Tsutomu Chiba, Jun Fujita |
Abstract |
There are a growing number of reports on the sub-physiological temperature culturing of mammalian cells for increased recombinant protein yields. However, the effect varies and the reasons for the enhancement are not fully elucidated. Expression of cold-inducible RNA-binding protein (cirp, also called cirbp or hnRNP A18) is known to be induced in response to mild, but not severe, hypothermia in mammalian cells. To clarify the molecular mechanism underlying the induction and to exploit this to improve the productivity of recombinant proteins, we tried to identify the regulatory sequence(s) in the 5' flanking region of the mouse cirp gene. |
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