You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
The Proteome of Biologically Active Membrane Vesicles from Piscirickettsia salmonis LF-89 Type Strain Identifies Plasmid-Encoded Putative Toxins
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, September 2017
|
| DOI | 10.3389/fcimb.2017.00420 |
| Pubmed ID | |
| Authors |
Cristian Oliver, Mauricio A. Hernández, Julia I. Tandberg, Karla N. Valenzuela, Leidy X. Lagos, Ronie E. Haro, Patricio Sánchez, Pamela A. Ruiz, Constanza Sanhueza-Oyarzún, Marcos A. Cortés, María T. Villar, Antonio Artigues, Hanne C. Winther-Larsen, Ruben Avendaño-Herrera, Alejandro J. Yáñez |
| Abstract |
Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 65 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 15% |
| Student > Bachelor | 10 | 15% |
| Student > Doctoral Student | 6 | 9% |
| Student > Master | 6 | 9% |
| Student > Ph. D. Student | 5 | 8% |
| Other | 9 | 14% |
| Unknown | 19 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 20% |
| Biochemistry, Genetics and Molecular Biology | 12 | 18% |
| Veterinary Science and Veterinary Medicine | 8 | 12% |
| Immunology and Microbiology | 5 | 8% |
| Medicine and Dentistry | 3 | 5% |
| Other | 1 | 2% |
| Unknown | 23 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 September 2017.
All research outputs
#17,916,739
of 23,003,906 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#4,160
of 6,498 outputs
Outputs of similar age
#229,773
of 321,004 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#72
of 97 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,498 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 321,004 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 97 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.