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Type 3 Secretion Systems

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Cover of 'Type 3 Secretion Systems'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Type III Secretion Systems.
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    Chapter 2 Site-Directed Mutagenesis and Its Application in Studying the Interactions of T3S Components.
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    Chapter 3 Blue Native Protein Electrophoresis to Study the T3S System Using Yersinia pestis as a Model.
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    Chapter 4 In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated Using the Yersinia pestis T3S System.
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    Chapter 5 Isolation of Type III Secretion System Needle Complexes by Shearing.
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    Chapter 6 Use of Transcriptional Control to Increase Secretion of Heterologous Proteins in T3S Systems.
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    Chapter 7 Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.
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    Chapter 8 Analysis of Type III Secretion System Secreted Proteins.
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    Chapter 9 Fractionation Techniques to Examine Effector Translocation.
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    Chapter 10 Measurement of Effector Protein Translocation Using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.
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    Chapter 11 A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas.
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    Chapter 12 Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.
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    Chapter 13 A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants.
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    Chapter 14 Detecting Immune Responses to Type III Secretion Systems.
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    Chapter 15 Recombinant Expression and Purification of the Shigella Translocator IpaB.
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    Chapter 16 Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.
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    Chapter 17 Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to These Proteins.
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    Chapter 18 Identification of the Targets of Type III Secretion System Inhibitors.
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    Chapter 19 Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis.
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