High Content Screening

Chapter 1 Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays

Chapter 2 General Staining and Segmentation Procedures for High Content Imaging and Analysis

Chapter 3 Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis

Chapter 4 Cell-Based High Content Analysis of Cell Proliferation and Apoptosis

Chapter 5 Tools to Measure Autophagy Using High Content Imaging and Analysis

Chapter 6 Guidelines for Microplate Selection in High Content Imaging

Chapter 7 Quality Control for High-Throughput Imaging Experiments Using Machine Learning in Cellprofiler

Chapter 8 High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening

Chapter 9 Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays

Chapter 10 Live-Cell High Content Screening in Drug Development

Chapter 11 Challenges and Opportunities in Enabling High-Throughput, Miniaturized High Content Screening

Chapter 12 Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells

Chapter 13 High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions

Chapter 14 High Content Imaging Assays for IL-6-Induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines

Chapter 15 Single Cell and Population Level Analysis of HCA Data

Chapter 16 Utilization of Multidimensional Data in the Analysis of Ultra-High-Throughput High Content Phenotypic Screens

Chapter 17 High Content Screening of Mammalian Primary Cortical Neurons

Chapter 18 Human-Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications

Chapter 19 Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays

Chapter 20 The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-Well Ultra-Low Attachment Microplates

Chapter 21 An Endothelial Cell/Mesenchymal Stem Cell Coculture Cord Formation Assay to Model Vascular Biology In Vitro

Chapter 22 High-Throughput Automated Chemical Screens in Zebrafish

Chapter 23 Erratum to: High Content Screening