| Chapter title |
Tools to Measure Autophagy Using High Content Imaging and Analysis
|
|---|---|
| Chapter number | 5 |
| Book title |
High Content Screening
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7357-6_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7355-2, 978-1-4939-7357-6
|
| Authors |
Nick J. Dolman, Brent A. Samson, Kevin M. Chambers, Michael S. Janes, Bhaskar S. Mandavilli |
| Abstract |
Macroautophagy, hereafter referred to as autophagy, is a predominately pro-survival catabolic process responsible for the degradation of long-lived or aggregated proteins, invading microorganisms and damaged or redundant intracellular organelles. Removal of these entities is achieved through encompassment of the target by the autophagosome and subsequent delivery to the lysosome. The use of fluorescence microscopy is a common method to investigate autophagy through monitoring the spatial and temporal recruitment both of autophagosomal markers and cargo to the autophagosome. In this section, we will discuss the use of high content imaging (HCI) and analysis in the study of autophagy with reference to commonly used markers of autophagosomal formation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Researcher | 2 | 33% |
| Student > Bachelor | 1 | 17% |
| Lecturer | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 33% |
| Business, Management and Accounting | 1 | 17% |
| Computer Science | 1 | 17% |
| Immunology and Microbiology | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
| Other | 0 | 0% |