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Chapter title |
Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells
|
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Chapter number | 12 |
Book title |
High Content Screening
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7357-6_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7355-2, 978-1-4939-7357-6
|
Authors |
Verena Fetz, Roland H. Stauber, Shirley K. Knauer |
Abstract |
In this chapter, you will learn how to use translocation biosensors to investigate protein functions in living cells. We here present three classes of modular protein translocation biosensors tailored to investigate: (1) signal-mediated nucleo-cytoplasmic transport, (2) protease activity, and (3) protein-protein interactions. Besides the mapping of protein function, the biosensors are also applicable to identify chemicals and/or (nano) materials modulating the respective protein activities and can also be exploited for RNAi-mediated genetic screens. |