Chapter 1 A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.

Chapter 2 Microscopy techniques to examine DNA replication in fission yeast.

Chapter 3 High-Resolution Analysis of Mammalian DNA Replication Units

Chapter 4 Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing

Chapter 5 Measuring DNA content by flow cytometry in fission yeast.

Chapter 6 Incorporation of thymidine analogs for studying replication kinetics in fission yeast.

Chapter 7 EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.

Chapter 8 Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.

Chapter 9 Measuring ribonucleotide incorporation into DNA in vitro and in vivo.

Chapter 10 Detection and Sequencing of Okazaki Fragments in S. cerevisiae.

Chapter 11 ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin.

Chapter 12 Chromatin immunoprecipitation to detect DNA replication and repair factors.

Chapter 13 Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism.

Chapter 14 Single-Molecule Observation of Prokaryotic DNA Replication

Chapter 15 Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.

Chapter 16 Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis

Chapter 17 Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes