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The CRAC Channel

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Cover of 'The CRAC Channel'

Table of Contents

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    Book Overview
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    Chapter 1 Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells
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    Chapter 2 Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai-Overexpressing HEK-293 Cells
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    Chapter 3 Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging
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    Chapter 4 Mn2+ Quenching Assay for Store-Operated Calcium Entry
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    Chapter 5 Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells Using Fluo-4 and Confocal Live-Cell Imaging
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    Chapter 6 Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations
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    Chapter 7 Indirect Measurement of CRAC Channel Activity Using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells
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    Chapter 8 CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR
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    Chapter 9 Western Blotting and Co-immunoprecipitation of Endogenous STIM/ORAI and Protein Partners
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    Chapter 10 Study of Endogenous CRAC Channels in Human Mast Cells Using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations
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    Chapter 11 Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures
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    Chapter 12 Study of the Endogenous CRAC Channel Using shRNA-Mediated Gene Silencing
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    Chapter 13 Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel
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    Chapter 14 Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI)
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    Chapter 15 High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy
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    Chapter 16 Single-Channel Single-Molecule Detection (SC-SMD) System
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