Chapter title |
Zebrafish
|
---|---|
Chapter number | 3 |
Book title |
Zebrafish
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3771-4_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
Authors |
Auer, Thomas O, Del Bene, Filippo, Thomas O. Auer, Filippo Del Bene, Auer, Thomas O., Bene, Filippo Del |
Abstract |
Targeting nucleases like zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) system have revolutionized genome-editing possibilities in many model organisms. They allow the generation of loss-of-function alleles by the introduction of double-strand breaks at defined sites within genes, but also more sophisticated genome-editing approaches have become possible. These include the integration of donor plasmid DNA into the genome by homology-independent repair mechanisms after CRISPR/Cas9-mediated cleavage. Here we present a protocol outlining the most important steps to target a genomic site and to integrate a donor plasmid at this defined locus. |
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Mendeley readers
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Other | 3 | 12% |
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Professor | 1 | 4% |
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Neuroscience | 1 | 4% |
Other | 1 | 4% |
Unknown | 6 | 24% |