| Chapter title |
The Nucleolus
|
|---|---|
| Chapter number | 6 |
| Book title |
The Nucleolus
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3792-9_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3790-5, 978-1-4939-3792-9
|
| Authors |
Martin, Robert M, Herce, Henry D, Ludwig, Anne K, Cardoso, M Cristina, Robert M. Martin, Henry D. Herce, Anne K. Ludwig, M. Cristina Cardoso |
| Editors |
Attila Németh |
| Abstract |
The nucleolus is the hallmark of nuclear compartmentalization and has been shown to exert multiple roles in cellular metabolism besides its main function as the place of ribosomal RNA synthesis and assembly of ribosomes. The nucleolus plays also a major role in nuclear organization as the largest compartment within the nucleus. The prominent structure of the nucleolus can be detected using contrast light microscopy providing an approximate localization of the nucleolus, but this approach does not allow to determine accurately the three-dimensional structure of the nucleolus in cells and tissues. Immunofluorescence staining with antibodies specific to nucleolar proteins albeit very useful is time consuming, normally antibodies recognize their epitopes only within a small range of species and is applicable only in fixed cells. Here, we present a simple method to selectively and accurately label this ubiquitous subnuclear compartment in living cells of a large range of species using a fluorescently labeled cell-penetrating peptide. |
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