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Mendeley readers
| Chapter title |
Fluorescence-Activated Nucleolus Sorting in Arabidopsis
|
|---|---|
| Chapter number | 15 |
| Book title |
The Nucleolus
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3792-9_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3790-5, 978-1-4939-3792-9
|
| Authors |
Frédéric Pontvianne, Myriam Boyer-Clavel, Julio Sáez-Vásquez |
| Editors |
Attila Németh |
| Abstract |
Nucleolar isolation allows exhaustive characterization of the nucleolar content. Centrifugation-based protocols are not adapted to isolation of nucleoli directly from a plant tissue because of copurification of cellular debris. We describe here a method that allows the purification of nucleoli using fluorescent-activated cell sorting from Arabidopsis thaliana leaves. This approach requires the expression of a specific nucleolar protein such as fibrillarin fused to green fluorescent protein in planta. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 33% |
| Lecturer | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 44% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Unknown | 3 | 33% |