Title |
Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure
|
---|---|
Published in |
Parasitology Research, October 2006
|
DOI | 10.1007/s00436-006-0326-5 |
Pubmed ID | |
Authors |
Giani F. Santoro, Maria das Graças Cardoso, Luiz Gustavo L. Guimarães, Ana Paula S. P. Salgado, Rubem F. S. Menna-Barreto, Maurilio J. Soares |
Abstract |
In the present work, we have investigated the effect of essential oils obtained from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) on growth and ultrastructure of diverse evolutive forms of Trypanosoma cruzi. Culture epimastigotes and bloodstream trypomastigotes were incubated for 24 h with different concentrations of oregano or thyme essential oils and with thymol (the main constituent of thyme), and the inhibitory concentration (IC)(50) was determined by cell counting. Crude extract of oregano essential oil inhibited epimastigote growth (IC(50)/24 h = 175 microg/ml) and also induced trypomastigote lysis (IC(50)/24 h = 115 microg/ml). Thyme essential oil presented IC(50)/24 h values of 77 microg/ml for epimastigotes and 38 mug/ml for trypomastigotes, while treatment with thymol resulted in an IC(50)/24 h of 62 microg/ml for epimastigotes and 53 microg/ml for trypomastigotes. Scanning electron microscopy of treated cells showed few morphological alterations at the plasma membrane. Observation by transmission electron microscopy showed cytoplasmic swelling with occasional morphological alterations in plasma and flagellar membrane. Our data indicate that oregano and thyme essential oils are effective against T. cruzi, with higher activity of thyme, and that thymol may be the main component responsible for the trypanocidal activity. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Colombia | 1 | <1% |
Italy | 1 | <1% |
Brazil | 1 | <1% |
Canada | 1 | <1% |
Mexico | 1 | <1% |
United States | 1 | <1% |
Unknown | 120 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 17% |
Student > Master | 18 | 14% |
Student > Bachelor | 17 | 13% |
Researcher | 16 | 13% |
Student > Doctoral Student | 12 | 10% |
Other | 16 | 13% |
Unknown | 25 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 44 | 35% |
Medicine and Dentistry | 11 | 9% |
Chemistry | 8 | 6% |
Biochemistry, Genetics and Molecular Biology | 5 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 4% |
Other | 18 | 14% |
Unknown | 35 | 28% |