| Chapter title |
A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells.
|
|---|---|
| Chapter number | 6 |
| Book title |
Wnt Signaling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6393-5_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6391-1, 978-1-4939-6393-5
|
| Authors |
Annette S. Flozak, Anna P. Lam M.D., Cara J. Gottardi Ph.D., Anna P. Lam, Cara J. Gottardi |
| Editors |
Quinn Barrett, Lawrence Lum |
| Abstract |
β-catenin (CTNNB1) is a dual-function cell-cell adhesion/transcriptional co-activator protein and an essential transducer of canonical Wnt signals. Although a number of established techniques and reagents are available to quantify the nuclear signaling activity of β-catenin (e.g., TCF-dependent reporter assays, nuclear accumulation of β-catenin, and generation of N-terminally hypophosphorylated β-catenin), there are cell-type and context-dependent limitations of these methods. Since the posttranscriptional stabilization of β-catenin outside of the cadherin complex appears universally required for β-catenin signaling, the following method allows for simple assessment of the cadherin-free fraction of β-catenin in cells, using a GST-tagged form of ICAT (Inhibitor of β-Catenin and Tcf) as an affinity matrix. This method is more sensitive and quantitative than immunofluorescence and may be useful in studies that implicate TCF-independent signaling events. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 22% |
| Student > Ph. D. Student | 3 | 13% |
| Other | 1 | 4% |
| Unknown | 14 | 61% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Agricultural and Biological Sciences | 3 | 13% |
| Medicine and Dentistry | 3 | 13% |
| Engineering | 1 | 4% |
| Unknown | 13 | 57% |