Computational Protein Design
Humana Press
Chapter title |
Production of Computationally Designed Small Soluble- and Membrane-Proteins: Cloning, Expression, and Purification.
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Chapter number | 3 |
Book title |
Computational Protein Design
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6637-0_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6635-6, 978-1-4939-6637-0
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Authors |
Barsa Tripathy, Rudresh Acharya |
Editors |
Ilan Samish |
Abstract |
This book chapter focuses on expression and purification of computationally designed small soluble proteins and membrane proteins that are ordinarily difficult to express in good amounts for experiments. Over-expression of such proteins can be achieved by using the solubility tag such as maltose binding protein (MBP), Thioredoxin (Trx), and Gultathione-S-transferase (GST) fused to the protein of interest. Here, we describe and provide the protocols for cloning, expression and purification of such proteins using the solubility tag. |
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