Production of Computationally Designed Small Soluble- and Membrane-Proteins: Cloning, Expression, and Purification.
Computational Protein Design
Methods in molecular biology, January 2017
Barsa Tripathy, Rudresh Acharya
This book chapter focuses on expression and purification of computationally designed small soluble proteins and membrane proteins that are ordinarily difficult to express in good amounts for experiments. Over-expression of such proteins can be achieved by using the solubility tag such as maltose binding protein (MBP), Thioredoxin (Trx), and Gultathione-S-transferase (GST) fused to the protein of interest. Here, we describe and provide the protocols for cloning, expression and purification of such proteins using the solubility tag.
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