↓ Skip to main content

Protein Blotting and Detection

Overview of attention for book
Cover of 'Protein Blotting and Detection'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Origins of protein blotting.
  3. Altmetric Badge
    Chapter 2 Protein Blotting and Detection
  4. Altmetric Badge
    Chapter 3 Introduction to protein blotting.
  5. Altmetric Badge
    Chapter 4 Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis.
  6. Altmetric Badge
    Chapter 5 Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.
  7. Altmetric Badge
    Chapter 6 Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometry.
  8. Altmetric Badge
    Chapter 7 Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time.
  9. Altmetric Badge
    Chapter 8 Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblots.
  10. Altmetric Badge
    Chapter 9 Slice blotting.
  11. Altmetric Badge
    Chapter 10 Localizing proteins by tissue printing.
  12. Altmetric Badge
    Chapter 11 Dot-immunobinding assay.
  13. Altmetric Badge
    Chapter 12 Capillary blotting of glycosaminoglycans on nitrocellulose membranes after agarose-gel electrophoresis separation.
  14. Altmetric Badge
    Chapter 13 Quantitative computerized western blotting.
  15. Altmetric Badge
    Chapter 14 Cationic electrophoresis and eastern blotting.
  16. Altmetric Badge
    Chapter 15 A miniaturized blotting system for simultaneous detecting of different autoantibodies.
  17. Altmetric Badge
    Chapter 16 Transfer and multiplex immunoblotting of a paraffin embedded tissue.
  18. Altmetric Badge
    Chapter 17 Multistrip western blotting to increase quantitative data output.
  19. Altmetric Badge
    Chapter 18 Western Blotting/Edman Sequencing using PVDF Membrane
  20. Altmetric Badge
    Chapter 19 Blotting from PhastGel to membranes by ultrasound.
  21. Altmetric Badge
    Chapter 20 Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heat.
  22. Altmetric Badge
    Chapter 21 Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane.
  23. Altmetric Badge
    Chapter 22 Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blot.
  24. Altmetric Badge
    Chapter 23 Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures.
  25. Altmetric Badge
    Chapter 24 Efficient electroblotting of very large proteins using a vertical agarose electrophoresis system.
  26. Altmetric Badge
    Chapter 25 Semi-dry protein transfer and immunodetection of P-selectin using an antibody to its C-terminal tag.
  27. Altmetric Badge
    Chapter 26 The multiple antigen blot assay: a simple, versatile and multipurpose immunoenzymatic technique.
  28. Altmetric Badge
    Chapter 27 Blotting from immobilized pH gradient gels: application to total cell lysates.
  29. Altmetric Badge
    Chapter 28 Immunoprecipitation-western blot for proteins of low abundance.
  30. Altmetric Badge
    Chapter 29 Native electrophoresis and western blot analysis: method and applications.
  31. Altmetric Badge
    Chapter 30 Grid-immunoblotting.
  32. Altmetric Badge
    Chapter 31 Sequential use of immunoblots for characterization of autoantibody specificities.
  33. Altmetric Badge
    Chapter 32 Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane.
  34. Altmetric Badge
    Chapter 33 Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.
  35. Altmetric Badge
    Chapter 34 Detection of protein-protein interactions by far-western blotting.
  36. Altmetric Badge
    Chapter 35 On-membrane tryptic digestion of proteins for mass spectrometry analysis.
  37. Altmetric Badge
    Chapter 36 Western blot analysis of adhesive interactions under fluid shear conditions: the blot rolling assay.
  38. Altmetric Badge
    Chapter 37 ELISPOT assay on membrane microplates.
  39. Altmetric Badge
    Chapter 38 A Brief Review of Other Notable Protein Blotting Methods
  40. Altmetric Badge
    Chapter 39 Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore.
  41. Altmetric Badge
    Chapter 40 Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation.
  42. Altmetric Badge
    Chapter 41 Fluorescent labeling of proteins and its application to SDS-PAGE and western blotting.
  43. Altmetric Badge
    Chapter 42 Whole and strip nitrocellulose membrane as well as a new-line-immunoblotting of antigen using the chemiluminescence technique.
  44. Altmetric Badge
    Chapter 43 Protein stains to detect antigen on membranes.
  45. Altmetric Badge
    Chapter 44 Use of non-radioactive detection method for north- and southwestern blot.
  46. Altmetric Badge
    Chapter 45 Immunoblotting using radiolabeled reagents for detection.
  47. Altmetric Badge
    Chapter 46 Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods.
  48. Altmetric Badge
    Chapter 47 "Rainbow" western blotting.
  49. Altmetric Badge
    Chapter 48 Multiple antigen detection (MAD) western blotting.
  50. Altmetric Badge
    Chapter 49 Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-.
  51. Altmetric Badge
    Chapter 50 Detection of La/SS-B by western blot using nanogold-tagged antibodies and silver enhancement.
  52. Altmetric Badge
    Chapter 51 Imaging systems for westerns: chemiluminescence vs. infrared detection.
  53. Altmetric Badge
    Chapter 52 A fluorescent codetection system for immunoblotting and proteomics through ECL-Plex and CyDye labeling.
  54. Altmetric Badge
    Chapter 53 Visualization of unstained protein bands on PVDF.
  55. Altmetric Badge
    Chapter 54 Improved protein detection using cold microwave technology.
  56. Altmetric Badge
    Chapter 55 TLC blot (far-eastern blot) and its applications.
  57. Altmetric Badge
    Chapter 56 A brief review of other notable protein detection methods on blots.
  58. Altmetric Badge
    Chapter 57 Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays.
Overall attention for this book and its chapters
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

twitter
1 X user
facebook
1 Facebook page
wikipedia
15 Wikipedia pages

Readers on

mendeley
386 Mendeley
citeulike
4 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Protein Blotting and Detection
Published by
Methods in molecular biology, January 2009
DOI 10.1007/978-1-59745-542-8
ISBNs
978-1-934115-73-2, 978-1-59745-542-8, 978-9-78-193411-7
Authors

Kurien, Biji T, Scofield, R. Hal

Editors

Biji T. Kurien, R. Hal Scofield

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 386 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 9 2%
Portugal 4 1%
Germany 2 <1%
Switzerland 2 <1%
Australia 2 <1%
Malaysia 2 <1%
Denmark 2 <1%
United Kingdom 2 <1%
Canada 2 <1%
Other 16 4%
Unknown 343 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 112 29%
Researcher 87 23%
Student > Bachelor 48 12%
Student > Master 45 12%
Student > Doctoral Student 23 6%
Other 57 15%
Unknown 14 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 213 55%
Biochemistry, Genetics and Molecular Biology 55 14%
Medicine and Dentistry 26 7%
Chemistry 19 5%
Engineering 11 3%
Other 33 9%
Unknown 29 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2023.
All research outputs
#6,507,210
of 23,485,296 outputs
Outputs from Methods in molecular biology
#1,920
of 13,156 outputs
Outputs of similar age
#41,811
of 172,099 outputs
Outputs of similar age from Methods in molecular biology
#57
of 168 outputs
Altmetric has tracked 23,485,296 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 13,156 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,099 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 168 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.