You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Isolation of Apoplast.
|
|---|---|
| Chapter number | 18 |
| Book title |
Isolation of Plant Organelles and Structures
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6533-5_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
| Authors |
Erik Andreasson, Kibrom B. Abreha, Svante Resjö |
| Editors |
Nicolas L. Taylor, A. Harvey Millar |
| Abstract |
The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 μg of apoplastic proteins can be obtained in a volume of 300 μL from five potato leaflets, with minimal contamination by non-apoplastic proteins. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 25% |
| Professor > Associate Professor | 2 | 25% |
| Researcher | 2 | 25% |
| Student > Ph. D. Student | 1 | 13% |
| Lecturer | 1 | 13% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Environmental Science | 1 | 13% |
| Unknown | 1 | 13% |