Chapter title |
Enrichment of the Plant Cytosolic Fraction.
|
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Chapter number | 17 |
Book title |
Isolation of Plant Organelles and Structures
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6533-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6531-1, 978-1-4939-6533-5
|
Authors |
Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, Joshua L. Heazlewood, Lao, Jeemeng, Smith-Moritz, Andreia M., Mortimer, Jennifer C., Heazlewood, Joshua L. |
Editors |
Nicolas L. Taylor, A. Harvey Millar |
Abstract |
The cytosol is at the core of cellular metabolism and contains many important metabolic pathways, including glycolysis, gluconeogenesis, and the pentose phosphate pathway. Despite the importance of this matrix, few attempts have sought to specifically enrich this compartment from plants. Although a variety of biochemical pathways and signaling cascades pass through the cytosol, much of the focus has usually been targeted at the reactions that occur within membrane-bound organelles of the plant cell. In this chapter, we outline a method for the enrichment of the cytosol from rice suspension cell cultures which includes sample preparation and enrichment as well as validation using immunoblotting and fluorescence-tagged proteins. |
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United States | 1 | 100% |
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Members of the public | 1 | 100% |