| Chapter title |
Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage, and Cell Death Following Exposure of Macrophages to Silica Particles.
|
|---|---|
| Chapter number | 5 |
| Book title |
Phagocytosis and Phagosomes
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6581-6_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6579-3, 978-1-4939-6581-6
|
| Authors |
Gaurav N. Joshi, Renée M. Gilberti, David A. Knecht |
| Editors |
Roberto Botelho |
| Abstract |
Chronic inhalation of silica in various occupational settings results in the development of silicosis, a disease characterized by lung fibrosis. Uptake of silica particles by alveolar macrophages results in cell death and this is one of the contributing factors to the development of silicosis. We have characterized the uncoated or protein-coated (non-opsonized) and Fc receptor-mediated (antibody-opsonized) routes of silica phagocytosis and toxicity. Numerous microscopy techniques and fluorescent probes are outlined in this chapter to carefully measure particle uptake, by macrophages, phagosome maturation, phagosomal reactive oxygen species generation, phagolysosomal leakage, and cell death. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 3 | 27% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Postgraduate | 2 | 18% |
| Professor > Associate Professor | 1 | 9% |
| Other | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 3 | 27% |
| Agricultural and Biological Sciences | 1 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Chemistry | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 4 | 36% |