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Chapter title |
Quantitative Spatiotemporal Analysis of Phagosome Maturation in Live Cells.
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Chapter number | 11 |
Book title |
Phagocytosis and Phagosomes
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6581-6_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6579-3, 978-1-4939-6581-6
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Authors |
Laura Schnettger, Maximiliano G. Gutierrez |
Editors |
Roberto Botelho |
Abstract |
Phagocytosis and phagosome maturation are central to the development of the innate and adaptive immune response. Phagosome maturation is a continuous and dynamic process that occurs rapidly. In this chapter, we describe fluorescence-based live cell imaging methods for the quantitative and temporal analysis of phagosome maturation of latex beads and M. tuberculosis as two phagocytic targets. We also describe two simple protocols for monitoring phagosome maturation: the use of the acidotropic probe LysoTracker and analyzing the recruitment of EGFP-tagged host proteins by phagosomes. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 40% |
Researcher | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Nursing and Health Professions | 1 | 20% |
Unknown | 2 | 40% |