| Chapter title |
Phospho-Flow Analysis of Primary Mouse Cells After HDAC Inhibitor Treatment
|
|---|---|
| Chapter number | 17 |
| Book title |
HDAC/HAT Function Assessment and Inhibitor Development
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6527-4_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6525-0, 978-1-4939-6527-4
|
| Authors |
Dagmar Hildebrand, Katharina F. Kubatzky |
| Abstract |
Flow cytometric techniques allow fast, sensitive, and multiparametric analyses at the single cell level. This makes it possible to distinguish subsets of cells within heterogeneous samples. Moreover, flow cytometry has become a frequently used method for the evaluation of therapeutic effects. Here, we describe the analysis of the phosphorylation status of signal transducer and activator of transcription-1 (STAT-1) in primary mouse cells after treatment with histone deacetylase inhibitors (HDACi) that are currently considered anticancer agents. We provide detailed protocols for the preparation of murine bone marrow cells and the staining of HDACi-treated cells, as well as an insight into the concepts of flow cytometry analysis. |
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