Chapter title |
Real-Time Analysis of the Apical Hook Development.
|
---|---|
Chapter number | 1 |
Book title |
Plant Hormones
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6469-7_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6467-3, 978-1-4939-6469-7
|
Authors |
Qiang Zhu, Petra Žádníková, Dajo Smet, Dominique Van Der Straeten, Eva Benková |
Editors |
Jürgen Kleine-Vehn, Michael Sauer |
Abstract |
Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process. |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
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Professor > Associate Professor | 2 | 25% |
Researcher | 1 | 13% |
Unknown | 3 | 38% |
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Agricultural and Biological Sciences | 3 | 38% |
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Unknown | 3 | 38% |