Plant Hormones
Springer New York
Chapter title |
Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin.
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Chapter number | 12 |
Book title |
Plant Hormones
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6469-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6467-3, 978-1-4939-6469-7
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Authors |
Siobhan A. Braybrook, Braybrook, Siobhan A, Braybrook, Siobhan A. |
Editors |
Jürgen Kleine-Vehn, Michael Sauer |
Abstract |
Atomic force microscopy, and related nano-indentation techniques, is a valuable tool for analyzing the elastic properties of plant cell walls as they relate to changes in cell wall chemistry, changes in development, and response to hormones. Within this chapter I will describe a method for analyzing the effect of the phytohormone auxin on the cell wall elasticity of tobacco BY-2 cells. This general method may be easily altered for different experimental systems and hormones of interest. |
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