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| Chapter title |
Multiplex Analysis Using cDNA Transcriptomic Profiling.
|
|---|---|
| Chapter number | 9 |
| Book title |
Multiplex Biomarker Techniques
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6730-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6729-2, 978-1-4939-6730-8
|
| Authors |
Steve F. C. Hawkins, Paul C. Guest |
| Editors |
Paul C. Guest |
| Abstract |
DNA microarrays contain microscopic DNA spots attached to a solid surface. Each spot contains picomolar levels of a specific DNA probe sequence and hybridization to the corresponding gene products can be detected and quantitated through the use of fluorescently labeled target DNA. In this format, DNA microarrays can be used to measure the expression level of thousands of genes in a single experiment. Here, we present a method to detect the mRNA transcriptional changes in neuronal precursor cells following differentiation using high density cDNA microarrays. |