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Mendeley readers
| Chapter title |
Multiplex Single Nucleotide Polymorphism Analyses.
|
|---|---|
| Chapter number | 10 |
| Book title |
Multiplex Biomarker Techniques
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6730-8_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6729-2, 978-1-4939-6730-8
|
| Authors |
Steve F. C. Hawkins, Paul C. Guest |
| Editors |
Paul C. Guest |
| Abstract |
Quantitative polymerase chain reaction (qPCR) is a routinely used method for detection and quantitation of gene expression in real time. This is achieved through the incorporation and measurement of fluorescent reporter probes in the amplified cDNA strands, since the fluorescent signals increase as the reaction progresses. The availability of multiple probes that fluoresce at different wavelengths allows for multiplexing as this gives rise to amplicons with unique fluorescent signatures. Here, we describe a method using the SensiFAST and SensiFAST One-Step probe kits which allows simultaneous real-time quantitation of up to 5 amplicons. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 1 | 100% |