Chapter title |
Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.
|
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Chapter number | 25 |
Book title |
Skeletal Muscle Stem Cells
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-3036-5_25 |
Pubmed ID | |
Book ISBNs |
978-1-07-163035-8, 978-1-07-163036-5
|
Authors |
Chen, Xiyue, Ferreira, Christina R, Kuang, Shihuan, Ferreira, Christina R. |
Abstract |
Lipid homeostasis is critical for maintaining normal cellular functions including membrane structural integrity, cell metabolism, and signal transduction. Adipose tissue and skeletal muscle are two major tissues involved in lipid metabolism. Adipose tissue can store excessive lipids in the form of triacylglyceride (TG), which can be hydrolyzed to release free fatty acids (FFAs) under insufficient nutrition states. In the highly energy-demanding skeletal muscle, lipids serve as oxidative substrates for energy production but can cause muscle dysfunction when overloaded. Lipids undergo fascinating cycles of biogenesis and degradation depending on physiological demands, while dysregulation of lipid metabolism has been increasingly recognized as a hallmark of diseases such as obesity and insulin resistance. Thus, it is important to understand the diversity and dynamics of lipid composition in adipose tissue and skeletal muscle. Here, we describe the use of multiple reaction monitoring profiling, based on lipid class and fatty acyl chain specific fragmentation, to explore various classes of lipids in skeletal muscle and adipose tissues. We provide a detailed method for exploratory analysis of acylcarnitine (AC), ceramide (Cer), cholesteryl ester (CE), diacylglyceride (DG), FFA, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS), sphingomyelin (SM), and TG. Characterization of lipid composition within adipose tissue and skeletal muscle under different physiological situations will provide biomarkers and therapeutic targets for obesity-related diseases. |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
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Unknown | 6 | 100% |
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Student > Doctoral Student | 1 | 17% |
Student > Bachelor | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
Student > Postgraduate | 1 | 17% |
Other | 0 | 0% |
Unknown | 1 | 17% |
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Veterinary Science and Veterinary Medicine | 1 | 17% |
Chemistry | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 1 | 17% |