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Skeletal Muscle Stem Cells

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Cover of 'Skeletal Muscle Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells
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    Chapter 2 Extra Eyelid-Derived Muscle Stem Cells
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    Chapter 3 Isolation, Culture, and Analysis of Zebrafish Myofibers and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis
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    Chapter 4 The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation Potential
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    Chapter 5 Co-cultures of Macrophages with Muscle Stem Cells with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle
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    Chapter 6 Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Muscle Stem Cells Using a Seahorse Analyzer: Applicability for Aging Studies
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    Chapter 7 High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes.
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    Chapter 8 State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts
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    Chapter 9 Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing
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    Chapter 10 In Vitro Maturation of Human Pluripotent Stem Cell-Derived Myotubes
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    Chapter 11 Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells
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    Chapter 12 Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells
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    Chapter 13 Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation
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    Chapter 14 Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Muscle
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    Chapter 15 Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading
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    Chapter 16 Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique
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    Chapter 17 Skeletal Muscle Regeneration in Zebrafish
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    Chapter 18 Methods to Monitor Circadian Clock Function in Skeletal Muscle
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    Chapter 19 Visualizing MyoD Oscillations in Muscle Stem Cells
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    Chapter 20 In Vivo Modeling of Skeletal Muscle Diseases Using the CRISPR/Cas9 System in Rats
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    Chapter 21 In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9-Mediated Genome Editing
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    Chapter 22 Exons 45–55 Skipping Using Antisense Oligonucleotides in Immortalized Human DMD Muscle Cells
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    Chapter 23 In Vivo Evaluation of Exon 51 Skipping in hDMD/Dmd-null Mice
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    Chapter 24 Functional Analysis of MicroRNAs in Skeletal Muscle
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    Chapter 25 Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.
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    Chapter 26 Single-Cell Transcriptomic Analysis of Mononuclear Cell Populations in Skeletal Muscle
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    Chapter 27 Assay for Transposase-Accessible Chromatin Using Sequencing of Freshly Isolated Muscle Stem Cells.
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    Chapter 28 Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.
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    Chapter 29 Epitranscriptome Mapping of N6-Methyladenosine Using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells.
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    Chapter 30 Visualization of RNA Transcripts in Muscle Stem Cells Using Single-Molecule Fluorescence In Situ Hybridization
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    Chapter 31 Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle
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    Chapter 32 Three-Dimensional Imaging Analysis for Skeletal Muscle
Attention for Chapter 28: Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.
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Chapter title
Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.
Chapter number 28
Book title
Skeletal Muscle Stem Cells
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-3036-5_28
Pubmed ID
Book ISBNs
978-1-07-163035-8, 978-1-07-163036-5
Authors

Ding, Dong, Braun, Thomas

Abstract

Adult muscle stem cells (MuSCs), also called satellite cells, are situated under the basal lamina of myofibers in skeletal muscles. MuSCs are instrumental for postnatal muscle growth and regeneration of skeletal muscles. Under physiological conditions, the majority of MuSCs is actively maintained in a quiescent state but becomes rapidly activated during muscle regeneration, which is accompanied with massive changes in the epigenome. Moreover, aging, but also pathological conditions, such as in muscle dystrophy, results in profound changes of the epigenome, which can be monitored with different approaches. However, a better understanding of the role of chromatin dynamics in MuSCs and its function for skeletal muscle physiology and disease has been hampered by technical limitations, mostly due to the relatively low number of MuSCs but also due to the strongly condensed chromatin state of quiescent MuSCs. Traditional chromatin immunoprecipitation (ChIP) usually requires large amounts of cells and has several other shortcomings. Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is a simple alternative to ChIP for chromatin profiling, providing higher efficiency and better resolution at lower costs. CUT&RUN maps genome-wide chromatin features, including genome-wide localization of transcription factor binding in small numbers of freshly isolated MuSCs, facilitating analysis of different subpopulations of MuSCs. Here we describe an optimized protocol to profile global chromatin in freshly isolated MuSCs using CUT&RUN.

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